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KMID : 1024519970060030249
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Journal of the Environmental Sciences
1997 Volume.6 No. 3 p.249 ~ p.258
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Lsolation and Characterization of Mercury- and Cadmium-resistant Bacteria isolated from Industrial complex Wastewater of Taejon Area
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You Kyung-Man
Jeon Hee-Keun
Park Joon-Bong
Lee In-Soo
Park Jin-Sook
Ha Nam-Joo
Park Yong-Keun
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Abstract
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Mercury- and cadmium-resistant bacteria were Isolated from an Industrial complex wastewater of Taejon area. All of them were motile, gram negative rods. In the results of physicochemical test and VITEK card test. HM1 was Identified with Achetobacter cd- coaceucus, CM3 was Identified 65 Commonas acidovorns, HM2, HM3, CMI , and CM4 were Pseudomonas sp., but HM4 and CM2 were unidenteed. They were tested for subceptlbility to 14 heavy metals. Mercury-resistant bacteria(HM1, HM2, HM3, and HM4) were sensitive to low concentration(100~400ppm) of , . , and Ni2+, while cadmium-resistant bacteria(CM1, CM2, CM3, and CM4) showed resistance up to the high concentration(600~ 1, 200ppm) of these metal loons. As a result of resistance spectrum test of mercury-resistant bacteria, HM1 was broad-spectrum strain, HM2. HM3, and HM4 were narrow-spectrum stratas. Transmission electron microscopic examination of cell wall of HM1 culture grown with and without 100ppm of showed remarkably merphological abnormalities. In the result of atomic absorption spectrometric analysis of cadmium-resistant bacteria grown at 200ppm of for 6h, all of them accumulated cadrnium(14ppm~57ppm) In cell. In cadinium-resistant bacteria, CM1, CM2, and CM4 were spared from the Inhibitory effect of by the addition of , CM4 were also spared from the Inhibitory effect of by the addition of as well as .
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KEYWORD
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mercury-resistant bacteria, cadmium-resistant bacteria, Acinetobacter cal-coaceticus, Comamonas acidovorans
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